This course will be held in Tartu (Estonia) next Aug 30-Sep 3, 2010. The practical course will include introduction to mass spectrometry and quantitative proteomics and then focus on two most popular techniques in the field - SILAC (stable isotope labeled amino acids in cell culture) and iTRAQ (isobaric tag for relative and absolute quantitation, Applied Biosystems). Topics covered will include experiment design, sample preparation, system setup and evaluation, sample analysis and data analysis. All MS analysis will be performed on an LTQ Orbitrap hybrid mass spectrometer (Thermo Electron), equipped with a nano-LC (Agilent 1200), nano-spray source and self-packed emitters (Proxeon). In addition, different protein and peptide fractionation techniques will be covered (isoelectric focusing, gel electrophoresis etc). This course is targeted for PhD students and post-docs from European Union. The course is free of charge. Organizers cover: - course materials - accommodation in Tartu in Hotel Dorpat, August 29 - September 4, 2010 - travel expenses (up to 400 EUR per person, larger expenses have to be agreed by the organizers) - meals (ex. dinner) - social events NB! The deadline for registration is until August 10, 2010

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