The International Proteomics Tutorial Programme


An Initiative to create an
Open-Access Teaching Resource

The most critical functions of the various Proteomics organisations are the training of young scientists and the dissemination of information to the general scientific community.  An International Tutorial Programme is therefore being launched to meet these needs*. It is a cooperation between selected journal publishers in the field of proteomics on the one hand, and HUPO and  its affiliated regional and national organisations. The programme is being organised by Peter James, Kazuyuki Nakamura and Thierry Rabilloud.

  • The overall level is aimed at Masters/PhD level students with good basic training in biology, biochemistry and mathematics / statistics.
  • The tutorials, which will be published in the following Journals: Journal of Proteome Research, Journal of Proteomics, Molecular and Cellular Proteomics, and Proteomics, will consist of a review/teaching article with an accompanying talk slide presentation for classroom teaching.
  • The tutorial program will cover core techniques and basics as an introduction to scientists new to the field.  A second series of more advanced topics focussing on the application of proteomics techniques to biological problem solving will be launched at a later date after the basic series is completed.
  • The entire series of articles and slides will be made freely available for teaching use at the Journals and Organisations home-pages**


The founding partners of the programme are the Human Proteome  Organization (HUPO) and the European Proteomics association (EuPA). It is expected that other regional proteomics organizations will be joining the programme and contribute in the future.


** All Illustrations used on this site come from the four Journals that publish the tutorials and the copyright belongs to them: Journal of Proteome Research, Journal of Proteomics, Molecular and Cellular Proteomics and Proteomics.


General Concepts behind the Programme


Two-Part Programme


TUTORIALS:  These are available as open-access articles in four of the main proteomics journals.

SLIDE SHOWS:  These are annotated Powerpoint articles for teaching and are available for download from the Journal home pages.

Tutorial Aims

  1. Introduce concepts
  2. Provide historical background
  3. Illustrate use in modern research

Provide a view to the future!

Reviews are the usual path in which information is collated and disseminated but usually at a highly specialist level.  There are a few Journals with an educational focus such as BAMBED (Biochemistry and Molecular Biology Education) but these are usually focussed on the teacher.  Thus there is a clear need for a educational resource that is targeted at attracting young scientists interested in entering the field as well as informing established researchers in other areas of the potential of proteomics in their work.  The tutorial program will consist of two parts, the review-like teaching articles and a complementary talk slide-show.  The concept of establishing an open access library of slide shows for teaching appears to be a completely novel one and one which can only help the field. 


Article & Slide Show Directory

IPTP1: De Novo sequencing and homology searching. Ma and Johnson

Review article:

IPTP2: Two-dimensional gel electrophoresis in proteomics: a tutorial. Rabilloud and Lelong

Review article:


IPTP3. Metabolic flux analysis and visualization. Toya, Kono, Arakawa and Tomita

Review article:

IPTP5. Fourier transform mass spectrometry. Scigelova, Hornshaw, Giannakopulos A, Makarov A

Review article:

IPTP6. Protein identification using MS/MS data. Cottrell

Review article:


IPTP8. Reactome pathway analysis to enrich biological discovery in proteomics data sets. Haw, Hermjakob, D'Eustachio and Stein

Review article:


IPTP9. Principles of electrospray ionization. Wilm.

Review article:



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